FAT32 is a kind of file system and the 32 has to do with how much each little part of the hard drive can hold, in terms of data allocation and depending on your configuration.
If you haven’t rewritten to the FAT32 drive you’re attempting to recover, you should be fine. Install FAT Data Recovery on the machine you’re going to use to scan the FAT drive.
DO NOT install FAT Data Recovery on the drive you’re trying to recover! FAT Data Recovery works with machines using Windows 2000, 2003, XP and Vista. If you’ve got XP on any of your other machines, you’ll be fine to install FAT Data Recovery.